Molecular cloning and expression analysis of a heat shock protein(Hsp90) gene from black tiger shrimp (Penaeus monodon)
Molecular Biology Report,2009,36(1):127-134
褰卞搷鍥犲瓙锛?.829
Abstract: The techniques of homology cloning and anchored PCR were used to clone the Hsp90 gene from black tiger shrimp. The full length cDNA of black tiger shrimp Hsp90 (btsHsp90) contained a 5鈥?untranslated region (UTR) of 72 bp, an ORF (open reading frame) of 2160 bp encoding a polypeptide of 720 amino acids with an estimated molecular mass of 83-kDa and a 3鈥?UTR of 288 bp. The sequence of the coding region showed 90 and 84% homology with that of the Chiromantes haematocheir and Homo sapiens, respectively. Conserved signature sequences of Hsp90 gene family were found in the btsHsp90 deduced amino acid sequence. The temporal expressions of Hsp90 gene were constitutively in the black tiger shrimp tissues including liver, ovary, muscle, brain stomach, and heart, and their levels were markedly enhanced after 30-min heat treatment at 37掳C. In ovarian maturation stages, the expression of btsHsp90 was strongest in the second stage, weaker in the fourth and first stage.
姹熶笘璐?閭变附鍗?鍛ㄥ彂鏋?榛勫缓鍗?閮鎯?鏉ㄩ摽
褰卞搷鍥犲瓙锛?.829
Abstract: The techniques of homology cloning and anchored PCR were used to clone the Hsp90 gene from black tiger shrimp. The full length cDNA of black tiger shrimp Hsp90 (btsHsp90) contained a 5鈥?untranslated region (UTR) of 72 bp, an ORF (open reading frame) of 2160 bp encoding a polypeptide of 720 amino acids with an estimated molecular mass of 83-kDa and a 3鈥?UTR of 288 bp. The sequence of the coding region showed 90 and 84% homology with that of the Chiromantes haematocheir and Homo sapiens, respectively. Conserved signature sequences of Hsp90 gene family were found in the btsHsp90 deduced amino acid sequence. The temporal expressions of Hsp90 gene were constitutively in the black tiger shrimp tissues including liver, ovary, muscle, brain stomach, and heart, and their levels were markedly enhanced after 30-min heat treatment at 37掳C. In ovarian maturation stages, the expression of btsHsp90 was strongest in the second stage, weaker in the fourth and first stage.
姹熶笘璐?閭变附鍗?鍛ㄥ彂鏋?榛勫缓鍗?閮鎯?鏉ㄩ摽
寰俊鎵竴鎵?br>鍒嗕韩鍒版湅鍙嬪湀
绮ゅ叕缃戝畨澶?4010502001742鍙?/a>